CITO Seminar: Joy Wolfram, PhD
Extracellular vesicles as therapeutic and diagnostic agents
Joy Wolfram, PhD
Assistant Professor of Medicine
Mayo Clinic - Jacksonville
Patients are dying every second! We need new and innovative solutions for early detection and treatment of diseases. As the director of the Nanomedicine and Extracellular Vesicles Laboratory at Mayo Clinic, my goal is to develop innovative nanomedicines that bring the next generation of cancer and regenerative treatments directly to the clinic. Extracellular vesicles (EVs) are naturally occurring nanoparticles that are secreted by all cells and serve important roles in physiological and pathological processes. Therefore, EVs have promising applications as therapeutic agents, drug targets, delivery vehicles, and biomarkers . Although the medical use of EVs is promising, clinical translation has been hindered due to inefficient and non-scalable methods of isolation, e.g. ultracentrifugation (UC). To overcome these issues, we have optimized a robust and scalable tangential flow filtration-based method for isolation of EVs. This technique has opened up many opportunities for diagnostic and therapeutic use of EVs, two of which will be highlighted in this talk: i) lipoaspirate-derived EVs for treating tissue damage and inflammation and ii) understanding the role and application of the EV glycome in metastatic disease.